What should be the melting temperature of a 10 base pair primer having 70% G+C content? - annealing temperature 70
I want to take this temperature as the annealing temperature for the RAPD-PCR.
Tuesday, February 9, 2010
Annealing Temperature 70 What Should Be The Melting Temperature Of A 10 Base Pair Primer Having 70% G+C Content?
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1 comments:
Handy-Dandy about this tool, discover the first performance. It makes sense:
http://www.promega.com/biomath/calc11.ht ...
It may also be more detailed than you need, but it is useful for numbers.
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